京尼平
胶原酶
角膜
眼科
极限抗拉强度
化学
医学
材料科学
生物化学
复合材料
壳聚糖
酶
作者
Marcel Y. Ávila,José Navia
出处
期刊:Journal of Cataract and Refractive Surgery
[Ovid Technologies (Wolters Kluwer)]
日期:2010-04-01
卷期号:36 (4): 659-664
被引量:91
标识
DOI:10.1016/j.jcrs.2009.11.003
摘要
PURPOSE: To evaluate the effect of genipin, a natural crosslinker, on porcine corneas. SETTING: Department of Ophthalmology, Universidad Nacional de Colombia, Bogota, Colombia. METHODS: Corneal strips (12.0 mm × 2.3 mm) were harvested from porcine eyes and treated by incubation with genipin at concentrations of 1.00%, 0.25%, and 0.10%. Parallel corneal strips from the same eye were used as untreated controls. After treatment at 20°C for 40 minutes, tensile strain measurements were performed in a biomaterial tester. Porcine button corneas were treated with genipin 0.25% for 15 minutes and then digested by bacterial collagenase. Treated and untreated corneas were evaluated by light microscopy. RESULTS: Young modulus and stiffness in treated corneas increased in a concentration-dependent manner. Genipin increased resistance to corneal collagenase 5-fold in comparison with the controls. A decrease in the interlamellar space in treated corneas was also observed. CONCLUSIONS: Corneal collagen crosslinking induced with genipin produced a significant increase in biomechanical strength and resistance to bacterial collagenase. This crosslinker could be useful in treating corneal ectasia and corneal infectious and noninfectious diseases involving corneal melting. Financial Disclosure: Neither author has a financial or proprietary interest in any material or method mentioned.
科研通智能强力驱动
Strongly Powered by AbleSci AI