Influence of milk proteins on lipid oxidation in aqueous emulsion: II. Lactoperoxidase, lactoferrin, superoxide dismutase and xanthine oxidase

乳过氧化物酶 化学 乳铁蛋白 黄嘌呤氧化酶 超氧化物歧化酶 脂质氧化 生物化学 黄嘌呤 氧化磷酸化 核化学 血红素 过氧化物酶 抗氧化剂 血红素
作者
John C. Allen,Wendy Wrieden
出处
期刊:Journal of Dairy Research [Cambridge University Press]
卷期号:49 (2): 249-263 被引量:39
标识
DOI:10.1017/s0022029900022354
摘要

SUMMARY The milk-related model system described by Allen & Wrieden (1982) was used to assess the effects of a number of purified milk metalloproteins, at concentrations relevant to those in milk and dairy products, on triglyceride oxidation in emulsions. Oxidations were monitored by the rate of O 2 utilization (ROU) and by the thiobarbituricacid (TBA) assay. The proteins lactoferrin, lactoperoxidase, superoxide dismutase and xanthine oxidase were used either on their own or in conjunction with 10 μM-Cu 2+ or Fe 2+ . Lactoferrin alone had little effect on oxidation, although both the iron-free and iron-saturated protein were able to protect the lipid to some extent from Fe 2+ -catalysed oxidation. Cu 2+ -catalysed oxidation was slightly promoted. Lactoperoxidase was pro-oxidative in the absence and presence of added Cu 2+ or Fe 2+ . Heat treatment at 72°C/20 s had little effect, but oxidation was greatly reduced by treatment at 80°C/20 s. Superoxide dismutase strongly reduced the rate of lipid oxidation in the absence of added metals, but had no effect in the presence of 10 μM-Cu 2+ . xanthine oxidase had little effect on lipid oxidation in the absence of added metals, but was strongly pro-oxidative in the presence of 10 μ-Cu 2+ . Similar effects were observed down to 1 μM-Cu 2+ . The enzymic activity of xanthine oxidase was rapidly eliminated by 10 μM-Cu 2+ , but not by 10 μM-Fe 2+ . A moderate pro-oxidative effect was observed in the presence of 10 μM-Fe 2+ . Heat treatment of xanthine oxidase at 80°C/20 s reduced its lipid pro-oxidative effect in the presence of Cu 2+ more effectively than treatment at 72°C/20 s.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
卡卡西应助查查采纳,获得20
刚刚
下一秒微笑完成签到,获得积分10
刚刚
mzf发布了新的文献求助10
2秒前
2秒前
3秒前
许你人间一两风完成签到,获得积分10
3秒前
dream发布了新的文献求助10
3秒前
刘步遥发布了新的文献求助10
4秒前
4秒前
田様应助鱼羊鲜采纳,获得10
5秒前
5秒前
轻松的亦寒应助啊懂采纳,获得20
5秒前
MM发布了新的文献求助10
5秒前
6秒前
7秒前
7秒前
Ava应助mayberichard采纳,获得10
7秒前
852应助可靠紫青采纳,获得10
7秒前
方块块发布了新的文献求助10
7秒前
小二郎应助shinn采纳,获得20
8秒前
Rank完成签到,获得积分10
8秒前
A2QD关注了科研通微信公众号
9秒前
9秒前
10秒前
Pom发布了新的文献求助10
10秒前
卡卡西应助Cc采纳,获得30
10秒前
wjx发布了新的文献求助10
10秒前
张鑫发布了新的文献求助30
11秒前
11秒前
12秒前
乐乐应助张涛采纳,获得10
13秒前
韩凡发布了新的文献求助10
13秒前
朱之欣发布了新的文献求助10
13秒前
鱼羊鲜完成签到,获得积分10
13秒前
难过千易发布了新的文献求助10
14秒前
普鲁卡因发布了新的文献求助10
14秒前
小段完成签到 ,获得积分10
14秒前
14秒前
15秒前
15秒前
高分求助中
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
Effective Learning and Mental Wellbeing 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3975165
求助须知:如何正确求助?哪些是违规求助? 3519595
关于积分的说明 11198781
捐赠科研通 3255912
什么是DOI,文献DOI怎么找? 1798001
邀请新用户注册赠送积分活动 877343
科研通“疑难数据库(出版商)”最低求助积分说明 806298