623MO Machine learning-based prediction of germinal center, MYC/BCL2 double protein expressor status, and MYC rearrangement from whole slide images in DLBCL patients

Diffuse Large B-Cells Lymphoma (DLBCL) is the most common type of non-Hodgkin lymphoma in adults (30-40%). In the 2016 World Health Organization (WHO), DLBCL are classified into 3 molecular subtypes according to cell of origin (COO), germinal-center, activated B-cell like and unclassified, based on gene-expression profiling (GEP). In daily routine, COO classification is replaced by immunochemistry (IHC) stains using the Hans algorithm based on the expression of CD10, BCL6 and MUM1 proteins. In addition, co-expression of BCL2 and MYC proteins is of prognostic value and defines the double-protein expressors (DPE) subtypes, associated with worse prognosis.