OptoLacI: optogenetically engineered lactose operon repressor LacI responsive to light instead of IPTG

紫胶操纵子 Lac抑制因子 乳糖 生物 操纵子 大肠杆菌 抑制因子 光遗传学 生物化学 生物物理学 细胞生物学 基因 基因表达 神经科学
作者
Meizi Liu,Z W Li,Jianfeng Huang,Junjun Yan,Guoping Zhao,Yanfei Zhang
出处
期刊:Nucleic Acids Research [Oxford University Press]
卷期号:52 (13): 8003-8016 被引量:1
标识
DOI:10.1093/nar/gkae479
摘要

Abstract Optogenetics’ advancement has made light induction attractive for controlling biological processes due to its advantages of fine-tunability, reversibility, and low toxicity. The lactose operon induction system, commonly used in Escherichia coli, relies on the binding of lactose or isopropyl β-d-1-thiogalactopyranoside (IPTG) to the lactose repressor protein LacI, playing a pivotal role in controlling the lactose operon. Here, we harnessed the light-responsive light-oxygen-voltage 2 (LOV2) domain from Avena sativa phototropin 1 as a tool for light control and engineered LacI into two light-responsive variants, OptoLacIL and OptoLacID. These variants exhibit direct responsiveness to light and darkness, respectively, eliminating the need for IPTG. Building upon OptoLacI, we constructed two light-controlled E. coli gene expression systems, OptoE.coliLight system and OptoE.coliDark system. These systems enable bifunctional gene expression regulation in E. coli through light manipulation and show superior controllability compared to IPTG-induced systems. We applied the OptoE.coliDark system to protein production and metabolic flux control. Protein production levels are comparable to those induced by IPTG. Notably, the titers of dark-induced production of 1,3-propanediol (1,3-PDO) and ergothioneine exceeded 110% and 60% of those induced by IPTG, respectively. The development of OptoLacI will contribute to the advancement of the field of optogenetic protein engineering, holding substantial potential applications across various fields.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
刚刚
1秒前
1秒前
1秒前
清璃发布了新的文献求助10
2秒前
wang发布了新的文献求助10
2秒前
懒YY捉小J完成签到 ,获得积分10
2秒前
文静的电灯胆完成签到,获得积分10
3秒前
3秒前
小赵完成签到,获得积分10
3秒前
传奇3应助能干的邹采纳,获得10
4秒前
夏下关注了科研通微信公众号
4秒前
41完成签到,获得积分10
4秒前
CipherSage应助清和采纳,获得10
4秒前
4秒前
张小哥12发布了新的文献求助10
4秒前
5秒前
xing完成签到,获得积分10
5秒前
white33c发布了新的文献求助10
6秒前
Tangjia发布了新的文献求助150
6秒前
6秒前
6秒前
6秒前
马明旋发布了新的文献求助10
7秒前
艾霙玥完成签到,获得积分20
7秒前
7秒前
李健应助碧蓝的汽车采纳,获得10
7秒前
小泓完成签到,获得积分10
7秒前
VV完成签到,获得积分10
8秒前
8秒前
123456发布了新的文献求助10
8秒前
9秒前
9秒前
只谈风月完成签到,获得积分10
10秒前
10秒前
善学以致用应助辛夷采纳,获得10
10秒前
10秒前
特土完成签到,获得积分10
10秒前
10秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Picture Books with Same-sex Parented Families: Unintentional Censorship 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 360
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3970632
求助须知:如何正确求助?哪些是违规求助? 3515261
关于积分的说明 11177794
捐赠科研通 3250448
什么是DOI,文献DOI怎么找? 1795314
邀请新用户注册赠送积分活动 875781
科研通“疑难数据库(出版商)”最低求助积分说明 805073