已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

PRRX1+MSCs Enhance Mandibular Regeneration during Distraction Osteogenesis

牙科 牵张成骨 再生(生物学) 口腔正畸科 化学 细胞生物学 生物 医学 分散注意力 神经科学
作者
Weidong Jiang,Peiqi Zhu,Tianming Zhang,Fangping Liao,Pengfei Jiang,Nuo Zhou,Xudong Wang,Xia Huang
出处
期刊:Journal of Dental Research [SAGE Publishing]
卷期号:102 (9): 1058-1068 被引量:9
标识
DOI:10.1177/00220345231176522
摘要

Bone defect (BD) caused by trauma, infection, congenital defects, or neoplasia is a major cause of physical limitation. Distraction osteogenesis (DO) is a highly effective procedure for bone regeneration, while the concrete mechanism remains unknown. In this study, canine DO and BD models of the mandible were established. The results of micro-computed tomography and histological staining revealed that DO led to an increased mineralized volume fraction and robust new bone formation; in contrast, BD demonstrated incomplete bone union. Mesenchymal stem cells (MSCs) from DO and BD calluses were isolated and identified. Compared with BD-MSCs, DO-MSCs were found to have a stronger osteogenic capability. Single-cell RNA sequencing analysis was further performed to comprehensively define cell differences between mandibular DO and BD calluses. Twenty-six clusters of cells representing 6 major cell populations were identified, including paired related homeobox 1-expressing MSCs (PRRX1+MSCs), endothelial cells (ECs), T cells, B cells, neutrophils, and macrophages. Interestingly, 2 subpopulations in PRRX1+MSCs in the DO group were found to express the marker of neural crest cells (NCCs) and were associated with the process of epithelial-mesenchymal transition. The immunofluorescence assay was performed to further corroborate these results in vivo and in vitro, experimentally validating that continuous distraction maintained the PRRX1+MSCs in an embryonic-like state. Finally, we used CRISPR/Cas9 to knock out (KO) PRRX1 in the context of DO, which significantly blunted the capability of jawbone regeneration, resulting in a diminished NCC-like program and reduction of new bone volume. In addition, the ability of osteogenesis, cell migration, and proliferation in cultured PRRX1KO MSCs was inhibited. Taken together, this study provides a novel, comprehensive atlas of the cell fates in the context of DO regeneration, and PRRX1+MSCs act essential roles.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
哈哈哈哈发布了新的文献求助10
1秒前
清爽源智发布了新的文献求助10
1秒前
nature发布了新的文献求助10
2秒前
善学以致用应助luwenxuan采纳,获得10
2秒前
似水流年完成签到 ,获得积分10
4秒前
Doki发布了新的文献求助10
5秒前
lutiantian发布了新的文献求助10
7秒前
酷波er应助瑾钰满糖采纳,获得10
8秒前
9秒前
卡皮巴拉完成签到,获得积分10
9秒前
清爽源智完成签到,获得积分10
12秒前
14秒前
漂亮采波完成签到,获得积分10
15秒前
YamDaamCaa应助科研顺利啦采纳,获得50
16秒前
敏感的南露完成签到,获得积分10
18秒前
Nefelibata完成签到,获得积分10
19秒前
bystanding完成签到,获得积分10
19秒前
慕青应助zty123采纳,获得10
21秒前
彪壮的银耳汤完成签到 ,获得积分10
23秒前
轩辕山槐完成签到,获得积分10
29秒前
30秒前
zty123完成签到,获得积分10
32秒前
大树完成签到 ,获得积分10
34秒前
34秒前
干净思远完成签到,获得积分10
34秒前
zty123发布了新的文献求助10
36秒前
领导范儿应助漂亮采波采纳,获得10
37秒前
量子星尘发布了新的文献求助10
37秒前
zxy完成签到 ,获得积分10
37秒前
科研通AI2S应助科研通管家采纳,获得30
38秒前
38秒前
Akim应助科研通管家采纳,获得10
38秒前
38秒前
38秒前
38秒前
38秒前
38秒前
852应助科研通管家采纳,获得10
38秒前
Limerencia完成签到,获得积分10
39秒前
echo完成签到 ,获得积分10
41秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3976600
求助须知:如何正确求助?哪些是违规求助? 3520674
关于积分的说明 11204422
捐赠科研通 3257298
什么是DOI,文献DOI怎么找? 1798683
邀请新用户注册赠送积分活动 877842
科研通“疑难数据库(出版商)”最低求助积分说明 806595