平移(音频)
噬菌体展示
多克隆抗体
抗体
重组DNA
计算生物学
生物
免疫学
生物化学
基因
古生物学
缩放
镜头(地质)
作者
Philip Alexander Heine,Maximilian Ruschig,Nora Langreder,Esther Veronika Wenzel,Maren Schubert,Federico Bertoglio,Michael Hust
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 261-274
被引量:2
标识
DOI:10.1007/978-1-0716-3381-6_13
摘要
Antibody phage display is a valuable in vitro technology to generate recombinant, sequence-defined antibodies for research, diagnostics, and therapy. Up to now (autumn 2022), 14 FDA/EMA-approved therapeutic antibodies were developed using phage display, including the world best-selling antibody adalimumab. Additionally, recombinant, sequence-defined antibodies have significant advantages over their polyclonal counterparts.For a successful in vitro antibody generation by phage display, a suitable panning strategy is highly important. We present in this book chapter the panning in solution and its advantages over panning with immobilized antigens and give detailed protocols for the panning and screening procedure.
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