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Comparative transcriptome and proteome reveal the unique genes and proteins of female parasitic wasps, Lysiphlebia japonica Ashmead

生物 转录组 粳稻 气味结合蛋白 棉蚜 蛋白质组 茧蜂科 植物 寄生蜂 基因 蚜虫科 遗传学 基因表达 有害生物分析 膜翅目 同翅目
作者
Jinming Li,Tingting Zhou,Xiangzhen Zhu,Li Wang,Kaixin Zhang,Dongyang Li,Jichao Ji,Junyu Luo,Jinjie Cui,Xueke Gao
出处
期刊:Pest Management Science [Wiley]
卷期号:80 (3): 1266-1278 被引量:1
标识
DOI:10.1002/ps.7856
摘要

Abstract BACKGROUND Lysiphlebia japonica Ashmead (Hymenoptera, Braconidae) is an endophagous parasitoid wasp and its host, Aphis gossypii Glover (Hemiptera, Aphididae) is a major cotton pest. L. japonica affects the growth and fatty acid metabolism of cotton aphids after parasitization and has been widely used as a biocontrol agent. However, there are currently few reports about the molecular characteristics of L. japonica , especially the differences between male and female. RESULTS In this study, using transcriptome and proteome analysis of the abdomen of female and male parasitic wasps, respectively, we obtained a total of 27,169 DEGs and 1,194 DEPs, then a total of 909 positively correlated high‐expression proteins and genes were obtained by combined omics analysis. Subsequently, 20 differentially expressed abdomen specific proteins were selected for validation by RT‐qPCR and Multiple Reaction Monitoring (MRM) protein verification. The result of RT‐qPCR demonstrated that all 20 genes were highly expressed in the abdomen of females, and five target proteins with unique peptide fragments and identification profiles were identified by MRM, which were venom protease, tropomyosin, lipase member I, venom serine carboxypeptidase and calreticulin, respectively. CONCLUSION Overall, these results provided molecular resources for the differences between males and females in L. japonica and the screened 20 abdomen specific proteins were verified to demonstrate the validity of the data, which offered important molecular data resources for further studies on the related functional genes of parasitic wasps and the mechanism of parasitoids regulating the growth of aphids. © 2023 Society of Chemical Industry.
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