In vivo biological analysis of cold plasma on allergenicity reduction of tropomyosin in shrimp

In vivo biological regulations of the allergenicity of tropomyosin (TM) treated by cold plasma (CP) were investigated by in vivo mouse model. The sensitization models of Balb/c mice were successfully established. CP treatment reduced the allergic symptoms of mice and regulated the Th1/Th2 balance to prevent allergy by activating Treg cells, which was deduced by serum and cytokines analysis. For intestinal flora analysis, allergy occurrence was accompanied by the decreased species abundance and the increased species diversity of intestinal flora. The significant species composition difference between the TM group and the PBS group showed a possible connection between bacterial diversity and allergy. Furthermore, Firmicutes, Bacteroidetes, Parabacteroides, Alloprevotella, Bacteroides, and Lachnospiraceae could relate to allergy occurrence. Intestinal section analysis suggested that allergy occurrence was accompanied by the damaged intestinal structure, and CP treatment could relieve the damage caused by an allergy.