基因组
人病毒体
计算生物学
生物
鉴定(生物学)
基因组
DNA测序
病毒
核酸
分离(微生物学)
新型病毒
生物信息学
遗传学
DNA
基因
生态学
作者
Cristian Bassi,Paola Guerriero,Marina Pierantoni,Elisa Callegari,Silvia Sabbioni
出处
期刊:Life
[MDPI AG]
日期:2022-12-07
卷期号:12 (12): 2048-2048
被引量:19
摘要
Metagenomic Next Generation Sequencing (mNGS) allows the evaluation of complex microbial communities, avoiding isolation and cultivation of each microbial species, and does not require prior knowledge of the microbial sequences present in the sample. Applications of mNGS include virome characterization, new virus discovery and full-length viral genome reconstruction, either from virus preparations enriched in culture or directly from clinical and environmental specimens. Here, we systematically reviewed studies that describe novel virus identification through mNGS from samples of different origin (plant, animal and environment). Without imposing time limits to the search, 379 publications were identified that met the search parameters. Sample types, geographical origin, enrichment and nucleic acid extraction methods, sequencing platforms, bioinformatic analytical steps and identified viral families were described. The review highlights mNGS as a feasible method for novel virus discovery from samples of different origins, describes which kind of heterogeneous experimental and analytical protocols are currently used and provides useful information such as the different commercial kits used for the purification of nucleic acids and bioinformatics analytical pipelines.
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