Single Cell Transcriptome Signatures of Sarcoidosis in Lung Immune Cell Populations

转录组 结节病 免疫系统 细胞 生物 计算生物学 免疫学 医学 病理 基因 基因表达 内科学 遗传学
作者
Camille M. Moore,Shu‐Yi Liao,Cheyret Wood,Abhimanyu Sarkar,Jonathan Cardwell,Kristyn MacPhail,Margaret M. Mroz,Christina Riley,Kara J. Mould,Clara I. Restrepo,Li Li,Lisa Maier,Ivana V. Yang
标识
DOI:10.1101/2025.01.20.633917
摘要

To identify cell specific molecular changes associated with sarcoidosis risk and progression, we aimed to characterize the cellular composition, gene expression patterns, and cell-cell interactions in BAL cells from patients with sarcoidosis (both progressive and non-progressive) and healthy controls. Single cell RNA-seq data were collected on 12 sarcoidosis and 4 control participants. We combined scRNA-seq data from these participants with our previously collected data on 4 sarcoidosis and 10 control participants for a final sample size of 16 sarcoidosis cases (8 progressive and 8 non-progressive) and 14 controls. Following initial preprocessing in CellRanger, data were quality controlled, combined, and clustered in Seurat. We tested differences in cell proportions by disease group using F-tests on cell proportions and differences in gene expression using pseudobulk analysis. Cell to cell communication and pathway analysis were performed using CellChat. We identified five macrophage populations: resident, high metallothionein (MT) resident, recruited, profibrotic recruited, and proliferating macrophages. Each subpopulation displayed unique gene expression profiles, with notable differential expression of genes and pathways linked to sarcoidosis in resident macrophages, recruited macrophages, and proliferating macrophages. We also observed changes in gene expression associated with disease progression in resident and recruited macrophages. In non-macrophages cells, we observed a significant reduction in the number of B cells in sarcoidosis patients compared to controls. Among T cell populations, we identified specific transcriptional alterations at gene and pathway level. Additionally, we observed distinct differences in cell-to-cell interactions of macrophages and T cells between sarcoidosis patients and healthy controls. These findings underscore the complexity of immune cell involvement in sarcoidosis and highlight potential cellular and molecular targets for further investigation.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
搜集达人应助ember采纳,获得10
刚刚
1秒前
2秒前
ayuelei发布了新的文献求助10
2秒前
Lucas应助sx采纳,获得10
3秒前
3秒前
崔雨旋完成签到,获得积分10
3秒前
3秒前
4秒前
4秒前
科研大满贯完成签到,获得积分10
4秒前
5秒前
5秒前
爱吃萝卜的Bob完成签到,获得积分10
6秒前
啵妞完成签到 ,获得积分10
6秒前
hulibin1208完成签到,获得积分10
6秒前
郁香薇发布了新的文献求助10
7秒前
hehe完成签到,获得积分10
7秒前
IrisFang1030完成签到,获得积分10
7秒前
7秒前
开心人达发布了新的文献求助10
8秒前
HPP123发布了新的文献求助10
8秒前
无情白羊发布了新的文献求助10
9秒前
10秒前
10秒前
dong应助ayuelei采纳,获得10
10秒前
Espionage发布了新的文献求助10
11秒前
11秒前
mmm发布了新的文献求助30
12秒前
上官又莲完成签到,获得积分10
12秒前
量子星尘发布了新的文献求助30
12秒前
Dana完成签到,获得积分10
12秒前
13秒前
英姑应助fafa采纳,获得10
14秒前
朝气发布了新的文献求助10
14秒前
烟花应助斤斤采纳,获得10
14秒前
hehe发布了新的文献求助10
16秒前
16秒前
隐形曼青应助what采纳,获得10
16秒前
asdfghjkl完成签到,获得积分10
17秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Picture Books with Same-sex Parented Families: Unintentional Censorship 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3969513
求助须知:如何正确求助?哪些是违规求助? 3514327
关于积分的说明 11173617
捐赠科研通 3249672
什么是DOI,文献DOI怎么找? 1794973
邀请新用户注册赠送积分活动 875537
科研通“疑难数据库(出版商)”最低求助积分说明 804836