清脆的
纳米技术
生物分析
纳米孔
纳米传感器
检出限
反式激活crRNA
小RNA
计算生物学
材料科学
化学
Cas9
生物
基因
色谱法
生物化学
作者
Bing Wang,Yi‐Tong Xu,Tianyang Zhang,Haiyan Wang,Xian Zhang,Zeng‐Qiang Wu,Weiwei Zhao,Hong‐Yuan Chen,Jing‐Juan Xu
出处
期刊:Nano Letters
[American Chemical Society]
日期:2023-12-21
卷期号:24 (1): 202-208
被引量:4
标识
DOI:10.1021/acs.nanolett.3c03723
摘要
This work presents a clustered regularly interspaced short palindromic repeat (CRISPR)/Cas-nanopipette nano-electrochemistry (Cas = CRISPR-associated proteins) capable of ultrasensitive microRNA detection. Nanoconfinement of the CRISPR/Cas13a within a nanopipette leads to a high catalytic efficacy of ca. 169 times higher than that in bulk electrolyte, contributing to the amplified electrochemical responses. CRISPR/Cas13a-enabled detection of representative microRNA-25 achieves a low limit of detection down to 10 aM. Practical application of this method is further demonstrated for single-cell and real human serum detection. Its general applicability is validated by addressing microRNA-141 and the SARS-CoV-2 RNA gene fragment. This work introduces a new CRISPR/Cas-empowered nanotechnology for ultrasensitive nano-electrochemistry and bioanalysis.
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