谷胱甘肽
化学
半胱氨酸
线粒体
活性氧
生物化学
抗氧化剂
GPX4
再灌注损伤
缺血
药理学
谷胱甘肽过氧化物酶
内科学
酶
生物
医学
作者
Jiayu Zeng,Haibo Liu,Ting Yang,Songjiao Li,Dan Cheng,Longwei He
出处
期刊:Talanta
[Elsevier]
日期:2024-04-01
卷期号:270: 125610-125610
被引量:5
标识
DOI:10.1016/j.talanta.2023.125610
摘要
Ferroptosis plays an important role in the early stage of myocardial ischemia/reperfusion (MI/R) injury, which is closely associated with the antioxidant damage of mitochondrial cysteine (Cys)/glutathione (GSH)/glutathione peroxidase 4 (GPX4) axis. Visualization of Cys and GSH in mitochondria is meaningful to value ferroptosis and further contributes to understanding and preventing MI/R injury. Herein a mitochondria-targetable thiols fluorescent probe (MTTP) was designed and synthesized based on sulfonyl benzoxadiazole (SBD) chromophore with a triphenylphosphine unit as the mitochondria-targeted functional group. Cys and GSH can be differentiated by MTTP with two distinguishable emission bands (583 nm and 520 nm) through the controllable aromatic substitution-rearrangement reaction. Importantly, MTTP is capable of monitoring ferroptosis and its inhibition by measuring mitochondrial Cys and GSH. MTTP was also employed to non-invasively detect ferroptosis during oxygen and glucose deprivation/reoxygenation (OGD/R)-induced MI/R injury in H9C2 cells. In a word, MTTP provides a visual tool that can simultaneously detect Cys and GSH to monitor ferroptosis processes during MI/R injury, which helps for more deeper understanding of the role of ferroptosis in MI/R injury-related diseases.
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