生物
外周血单个核细胞
诱导多能干细胞
SOX2
KLF4公司
髓系白血病
癌症研究
胚芽层
髓样
分子生物学
干细胞
胚胎干细胞
免疫学
遗传学
体外
基因
作者
Lihua Yuan,Manling Xie,Tao Yuan,Xi Chen,Xiaojun Xu,Sheng Wang
标识
DOI:10.1016/j.scr.2024.103360
摘要
We successfully developed an induced pluripotent stem cell (iPSC) line, SYSUSHi001-A, from the peripheral blood mononuclear cells (PBMC) of a patient with Acute Myeloid Leukemia, harboring two genetic mutations (XPO1: c.591-4_591-3dupTT; PALB2: c.3296C > T; p.T1099M). This iPSC line was facilitated through the use of episomal plasmids encoding OCT4, SOX2, KLF4, L-MYC, and human miR-302. The SYSUSHi001-A iPSC line exhibited characteristic embryonic stem cell-like morphology, maintained the XPO1 and PALB2 mutations, expressed key pluripotency markers, preserved a normal karyotype (46, XY), and demonstrated the ability to differentiate into cells from all three germ layers in vitro.
科研通智能强力驱动
Strongly Powered by AbleSci AI