Fluorinated trehalose analogues for cell surface engineering and imaging of Mycobacterium tuberculosis

The mycobacterial trehalose LpqY-SugABC ATP-binding cassette (ABC) transporter was hijacked to import fluorine-modified trehalose analogues to label cell-surface glycolipids and directly visualise the Mycobacterium tuberculosis pathogen by Focused Ion Beam (FIB) Secondary Ion Mass Spectrometry.