生物
生物发生
分离(统计)
生育率
细胞生物学
水泥
遗传学
基因
人口
材料科学
环境卫生
医学
机器学习
计算机科学
冶金
作者
Jie Gao,Jiongjie Jing,Guanyi Shang,Canmei Chen,Maoping Duan,Wenyang Yu,Sheng Wang,Jie Luo,Manxiu Song,Kun Chen,C. Chen,Tuo Zhang,Deqiang Ding
标识
DOI:10.1016/j.devcel.2024.06.017
摘要
The intermitochondrial cement (IMC) is a prominent germ granule that locates among clustered mitochondria in mammalian germ cells. Serving as a key platform for Piwi-interacting RNA (piRNA) biogenesis; however, how the IMC assembles among mitochondria remains elusive. Here, we identify that Tudor domain-containing 1 (TDRD1) triggers IMC assembly via phase separation. TDRD1 phase separation is driven by the cooperation of its tetramerized coiled-coil domain and dimethylarginine-binding Tudor domains but is independent of its intrinsically disordered region. TDRD1 is recruited to mitochondria by MILI and sequentially enhances mitochondrial clustering and triggers IMC assembly via phase separation to promote piRNA processing. TDRD1 phase separation deficiency in mice disrupts IMC assembly and piRNA biogenesis, leading to transposon de-repression and spermatogenic arrest. Moreover, TDRD1 phase separation is conserved in vertebrates but not in invertebrates. Collectively, our findings demonstrate a role of phase separation in germ granule formation and establish a link between membrane-bound organelles and membrane-less organelles.
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