外体
结直肠癌
癌胚抗原
生物标志物
微泡
微流控
癌症研究
癌症
纳米技术
医学
生物
材料科学
内科学
小RNA
基因
生物化学
作者
Peilong Li,Jiaci Chen,Yuqing Chen,Shangling Song,Xiaowen Huang,Yang Yang,Yanru Li,Yao Tong,Xie Yan,Juan Li,Shunxiang Li,Jiayi Wang,Kun Qian,Chuanxin Wang,Lutao Du
出处
期刊:Small
[Wiley]
日期:2023-02-17
卷期号:19 (20)
被引量:22
标识
DOI:10.1002/smll.202207381
摘要
Exosomes are promising new biomarkers for colorectal cancer (CRC) diagnosis, due to their rich biological fingerprints and high level of stability. However, the accurate detection of exosomes with specific surface receptors is limited to clinical application. Herein, an exosome enrichment platform on a 3D porous sponge microfluidic chip is constructed and the exosome capture efficiency of this chip is ≈90%. Also, deep mass spectrometry analysis followed by multi-level expression screenings revealed a CRC-specific exosome membrane protein (SORL1). A method of SORL1 detection by specific quantum dot labeling is further designed and the ensemble classification system is established by extracting features from 64-patched fluorescence images. Importantly, the area under the curve (AUC) using this system is 0.99, which is significantly higher (p < 0.001) than that using a conventional biomarker (carcinoembryonic antigen (CEA), AUC of 0.71). The above system showed similar diagnostic performance, dealing with early-stage CRC, young CRC, and CEA-negative CRC patients.
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