量子点
配体(生物化学)
材料科学
DNA
生物传感器
纳米技术
荧光
结合
自组装
纳米颗粒
猝灭(荧光)
组合化学
化学
量子力学
生物化学
物理
数学
数学分析
受体
作者
Paniz Rahmani,Melissa Goodlad,Yehan Zhang,Yichen Li,Tao Ye
标识
DOI:10.1021/acsami.2c10580
摘要
To address the current challenges in making bright, stable, and small DNA-functionalized quantum dots (QDs), we have developed a one-step ligand-exchange method to produce QD-DNA conjugates from commonly available hydrophobic QDs. We show that by systematically adjusting the reaction conditions such as ligand-to-nanoparticle molar ratio, pH, and solvent composition, stable and highly photoluminescent water-soluble QD-DNA conjugates with relatively high ligand loadings can be produced. Moreover, by site specifically binding these QD-DNA conjugates to a DNA origami template, we demonstrate that these bioconjugates have sufficient colloidal stability for DNA-directed self-assembly. Fluorescence quenching by an adjacent gold nanoparticle (AuNP) was demonstrated. Such QD-AuNP dimers may serve as biosensors with improved sensitivity and reproducibility. Moreover, our simple method can facilitate the assembly of QDs into more complex superlattices and discrete clusters that may enable novel photophysical properties.
科研通智能强力驱动
Strongly Powered by AbleSci AI