组胺
体内
刺激
细胞外
神经科学
体外
下丘脑
生物
皮质(解剖学)
生物胺
细胞生物学
生物物理学
化学
中枢神经系统
神经递质
生物化学
内分泌学
遗传学
作者
Hui Dong,Mengyao Li,Yuqi Yan,Tongrui Qian,Yunzhi Lin,Xiaoyuan Ma,Henry F. Vischer,Can Liu,Guochuan Li,Huan Wang,Rob Leurs,Yulong Li
标识
DOI:10.1101/2022.08.19.504485
摘要
Abstract Histamine (HA) is a key biogenic monoamine involved in a wide range of physiological and pathological processes in both the central nervous system and the periphery. Because the ability to directly measure extracellular HA in real-time will provide important insights into the functional role of HA in complex circuits under a variety of conditions, we developed a series of genetically encoded G protein-coupled r eceptor a ctivation- b ased (GRAB) HA (GRAB HA ) sensors. These sensors produce a robust increase in fluorescence upon HA application, with good photostability, sub-second kinetics, nanomolar affinity, and high specificity. Using these GRAB HA sensors, we measured electrical stimulation-evoked HA release in acute brain slices with high spatiotemporal resolution. Moreover, we recorded HA release in the preoptic area of the hypothalamus and in the medial prefrontal cortex during the sleep-wake cycle in freely moving mice, finding distinct patterns of HA release in these specific brain regions. Together, these in vitro and in vivo results show that our GRAB HA sensors have high sensitivity and specificity for measuring extracellular HA, thus providing a robust new set of tools for examining the role of HA signaling in both health and disease.
科研通智能强力驱动
Strongly Powered by AbleSci AI