Huaxiibacter chinensis gen. nov., sp. nov., recovered from human sputum

Strain 155047 T was recovered from human sputum in China in 2021. Preliminary species identification based on limited phenotypic tests assigned the strain to the genus Enterobacter of the family Enterobacteriaceae . The genome sequence of the strain was obtained and had ≤84.43 % average nucleotide identity (ANI) and ≤26.3 % in silico DNA–DNA hybridization (isDDH) values with the genomes of type strains of known Enterobacteriaceae species. The highest ANI and isDDH matches were with Lelliottia nimipressuralis and Enterobacter asburiae , respectively. The ANI and isDDH values support that the strain belongs to a novel species of the family Enterobacteriaceae . Phylogenomic analysis based on core genes revealed that strain 155047 T was located in the Enterobacter–Leclercia–Lelliottia–Pseudenterobacter lineage. The highest ANI and average amino acid identity values between 155047 T and any species of the Enterobacter–Leclercia–Lelliottia–Pseudenterobacter lineage were 84.43 % and 90.21 %, respectively, lower than the maximum inter-genus pairwise values. This indicates that 155047 T belongs to a novel species of a novel genus in the lineage. Strain 155047 T could be differentiated from Enterobacter , Lelliottia , Leclercia and Pseudenterobacter species by a negative reaction for β-galactosidase and the ability to produce acid from l -fucose but not from sucrose. The names Huaxiibacter gen. nov. and Huaxiibacter chinensis sp. nov. are proposed for the novel genus and species, respectively. The type strain is 155047 T (= GDMCC 1.2980 T =JCM 35262 T ).