Three-Dimensional Ovarian Organ Culture as a Tool to Study Normal Ovarian Surface Epithelial Wound Repair

器官培养 排卵 卵巢癌 卵巢 胎牛血清 细胞角蛋白 生物 男科 再生(生物学) 伤口愈合 上皮 波形蛋白 内分泌学 内科学 细胞培养 细胞生物学 癌症 免疫组织化学 医学 免疫学 体外 激素 生物化学 遗传学
作者
Kevin S. Jackson,Kari Inoue,David A. Davis,Tyvette S. Hilliard,Joanna E. Burdette
出处
期刊:Endocrinology [The Endocrine Society]
卷期号:150 (8): 3921-3926 被引量:26
标识
DOI:10.1210/en.2008-1674
摘要

Ovarian cancers are primarily derived from a single layer of epithelial cells surrounding the ovary, the ovarian surface epithelium (OSE). Ovarian surface proliferation is associated with ovulation and has been suggested to play a role in ovarian surface transformation and cancer progression. Aspects of ovarian surface repair after ovulation include proliferation, migration, and surface regeneration. To study ovarian surface repair, an organ culture system was developed that supports the proliferation, encapsulation, and repair of an artificially wounded surface. Wounded mouse ovaries embedded into an alginate hydrogel matrix have normal OSE cells as demonstrated by expression of cytokeratin 8, vimentin, N-cadherin, and a lack of E-cadherin. Normal OSE cells began proliferating and migrating around wounded surfaces after 1 d of culture. Organ cultures were propagated in medium supplemented with BSA and fetal bovine serum to determine optimal growth conditions. BSA cultured organs had OSE that proliferated significantly more than controls until d 4, whereas fetal bovine serum cultured organs had significantly more surface area encapsulated by OSE. Overall, a three-dimensional ovarian organ culture supports the growth of normal OSE in response to artificial wounding and provides a novel system for investigating wound repair as it relates to the possible role of ovulation and ovarian cancer.

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