微泡
泌尿系统
膜性肾病
蛋白质组学
肾小球基底膜
蛋白质组
病理
局灶节段性肾小球硬化
肾病
医学
肾小球肾炎
生物标志物
微小变化病
内科学
肾
内分泌学
生物
生物信息学
生物化学
糖尿病
基因
小RNA
作者
Ilse M. Rood,Michael L. Merchant,Daniel W. Wilkey,Terry Zhang,Vlad Zabrouskov,Johan van der Vlag,Henry Dijkman,Brigith Willemsen,Jack F.M. Wetzels,Jon B. Klein,Jeroen K. J. Deegens
出处
期刊:Proteomics
[Wiley]
日期:2015-09-28
卷期号:15 (21): 3722-3730
被引量:27
标识
DOI:10.1002/pmic.201500127
摘要
Urinary microvesicles constitute a rich source of membrane-bound and intracellular proteins that may provide important clues of pathophysiological mechanisms in renal disease. In the current study, we analyzed and compared the proteome of urinary microvesicles from patients with idiopathic membranous nephropathy (iMN), idiopathic focal segmental glomerulosclerosis (iFSGS), and normal controls using an approach that combined both proteomics and pathology analysis. Lysosome membrane protein-2 (LIMP-2) was increased greater than twofold in urinary microvesicles obtained from patients with iMN compared to microvesicles of patients with iFSGS and normal controls. Immunofluorescence analysis of renal biopsies confirmed our proteomics findings that LIMP-2 was upregulated in glomeruli from patients with iMN but not in glomeruli of diseased patients (iFSGS, minimal change nephropathy, IgA nephropathy, membranoproliferative glomerulonephritis) and normal controls. Confocal laser microscopy showed co-localization of LIMP-2 with IgG along the glomerular basement membrane. Serum antibodies against LIMP-2 could not be detected. In conclusion, our data show the value of urinary microvesicles in biomarker discovery and provide evidence for de novo expression of LIMP-2 in glomeruli of patients with iMN.
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