截形苜蓿
皂甙元
三萜
生物
糖基转移酶
蛇床子素
皂甙
生物合成
生物化学
莲藕
糖苷
苜蓿
基因
遗传学
植物
医学
病理
突变体
共生
替代医学
细菌
作者
Marina Naoumkina,Luzia V. Modolo,David V. Huhman,Ewa Urbańczyk-Wochniak,Yuhong Tang,Lloyd W. Sumner,Richard A. Dixon
出处
期刊:The Plant Cell
[Oxford University Press]
日期:2010-03-01
卷期号:22 (3): 850-866
被引量:172
标识
DOI:10.1105/tpc.109.073270
摘要
Abstract Saponins, an important group of bioactive plant natural products, are glycosides of triterpenoid or steroidal aglycones (sapogenins). Saponins possess many biological activities, including conferring potential health benefits for humans. However, most of the steps specific for the biosynthesis of triterpene saponins remain uncharacterized at the molecular level. Here, we use comprehensive gene expression clustering analysis to identify candidate genes involved in the elaboration, hydroxylation, and glycosylation of the triterpene skeleton in the model legume Medicago truncatula. Four candidate uridine diphosphate glycosyltransferases were expressed in Escherichia coli, one of which (UGT73F3) showed specificity for multiple sapogenins and was confirmed to glucosylate hederagenin at the C28 position. Genetic loss-of-function studies in M. truncatula confirmed the in vivo function of UGT73F3 in saponin biosynthesis. This report provides a basis for future studies to define genetically the roles of multiple cytochromes P450 and glycosyltransferases in triterpene saponin biosynthesis in Medicago.
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