邻苯二甲酸盐
色谱法
化学
增塑剂
液相色谱-质谱法
串联质谱法
邻苯二甲酸
邻苯二甲酸二乙酯
同位素稀释
尿
质谱法
生物化学
有机化学
作者
Yuan-Jhe Chang,Keh‐Liang Lin,Yan‐Zin Chang
标识
DOI:10.1016/j.cca.2012.10.009
摘要
Di-(2-ethylhexyl)phthalate (DEHP) is an endocrine disrupting chemical that is widely used as the major plasticizer for worldwide plastic products. It can cause several toxic effects to human with high dose exposure. In response to the need of human exposure assessment, different biological specimens are taken into account. Compared to blood, urine and other specimens, hair is unique in that it could determine the time period of chemical exposure after several months to years.The developed method consists of solution incubation, liquid-liquid extraction and stable isotope dilution liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.A reliable and sensitive analytical method was developed and validated for the determination of 5 metabolites, mono-(2-ethylhexyl)phthalate (MEHP), mono-(2-ethyl-5-hydroxyhexyl)phthalate (MEHHP), mono-(2-ethyl-5-oxy-hexyl)phthalate (MEOHP), mono-(2-ethyl-5-carboxypentyl)phthalate (5cx-MEPP) and mono-[2-(carboxymethyl)hexyl]phthalate (2cx-MMHP) in human hair. Ten authentic hair specimens were successfully determined and quantitated by the developed method.The developed LC-MS/MS method can successfully determine specific DEHP metabolites in human hair and has a great potential to assess the long term DEHP exposure of human.
科研通智能强力驱动
Strongly Powered by AbleSci AI