A novel mitochondria-targeted near-infrared fluorescence probe for ultrafast and ratiometric detection of SO 2 derivatives in live cells

A novel mitochondria-targeted ratiometric near-infrared fluorescence probe NDMBT for Sulfur dioxide (SO2) derivatives was constructed based on the SO2 derivatives-triggered Michael addition reaction. It displayed ultrafast response time (within 10 s), large hypsochromic shift (260 nm), high photostability, excellent selectivity and high sensitivity in aqueous media with a detection limit of 43 nM. More importantly, it was successfully applied to imaging of the enzymatically generated SO2 derivatives in mitochondria of live cells.