亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Establishment and characterization of uterine sarcoma and carcinosarcoma patient-derived xenograft models

癌肉瘤 医学 细胞角蛋白 肉瘤 波形蛋白 免疫组织化学 病理 肉瘤样癌 癌症研究 平滑肌肉瘤
作者
Tine Cuppens,Jeroen Depreeuw,Daniela Annibali,Debby Thomas,Els Hermans,Ellen Gommé,Xuan Bich Trinh,David Debruyne,Philippe Moerman,Diether Lambrechts,Frédéric Amant
出处
期刊:Gynecologic Oncology [Elsevier BV]
卷期号:146 (3): 538-545 被引量:16
标识
DOI:10.1016/j.ygyno.2017.06.005
摘要

Uterine sarcomas (US) and carcinosarcomas (CS) are rare, aggressive cancers. The lack of reliable preclinical models hampers the search for new treatment strategies and predictive biomarkers. To this end, we established and characterized US and CS patient-derived xenograft (PDX) models.Tumor fragments of US and CS were subcutaneously implanted into immunocompromised mice. Engrafted xenograft and original tumors were compared by means of histology, immunohistochemistry, whole-genome low-coverage sequencing for copy number variations, and RNA sequencing.Of 13 implanted leiomyosarcomas (LMS), 10 engrafted (engraftment rate of 77%). Also 2 out of 7 CS (29%) and one high-grade US (not otherwise specified) models were successfully established. LMS xenografts showed high histological similarity to their corresponding human tumors. Expression of desmin and/or H-caldesmon was detected in 8/10 LMS PDX models. We noticed that in CS models, characterized by the concomitant presence of a mesenchymal and an epithelial component, the relative distribution of the components is varying over the generations, as confirmed by changes in vimentin and cytokeratin expression. The similarity in copy number profiles between original and xenograft tumors ranged from 57.7% to 98.2% for LMS models and from 47.4 to 65.8% for CS models. Expression pattern stability was assessed by clustering RNA expression levels of original and xenograft tumors. Six xenografts clustered together with their original tumor, while 3 (all LMS) clustered apart.We present here a panel of clinically annotated uterine sarcoma and carcinosarcoma PDX models, which will be a useful tool for preclinical testing of new therapies.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Orange应助Boren采纳,获得10
14秒前
34秒前
Boren发布了新的文献求助10
38秒前
大个应助Boren采纳,获得10
50秒前
Raunio完成签到,获得积分10
52秒前
Akitten发布了新的文献求助10
1分钟前
完美世界应助lhy采纳,获得10
1分钟前
科研通AI2S应助科研通管家采纳,获得10
1分钟前
2分钟前
2分钟前
2分钟前
Boren发布了新的文献求助10
2分钟前
lhy发布了新的文献求助10
2分钟前
董可以发布了新的文献求助10
2分钟前
风吹而过完成签到 ,获得积分10
2分钟前
乐乐应助董可以采纳,获得10
2分钟前
2分钟前
3分钟前
liufan完成签到 ,获得积分10
3分钟前
科研通AI2S应助科研通管家采纳,获得10
3分钟前
打打应助科研通管家采纳,获得30
3分钟前
科研通AI2S应助科研通管家采纳,获得10
3分钟前
3分钟前
3分钟前
杪夏二八完成签到 ,获得积分10
4分钟前
souther完成签到,获得积分0
4分钟前
乐乐应助Akitten采纳,获得10
4分钟前
5分钟前
kangkang发布了新的文献求助10
5分钟前
传奇完成签到 ,获得积分10
5分钟前
jyy关闭了jyy文献求助
6分钟前
丘比特应助怕孤单的思雁采纳,获得10
6分钟前
6分钟前
6分钟前
Akitten发布了新的文献求助10
6分钟前
6分钟前
Li完成签到,获得积分10
6分钟前
7分钟前
自然的衫完成签到 ,获得积分10
7分钟前
7分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990332
求助须知:如何正确求助?哪些是违规求助? 3532146
关于积分的说明 11256483
捐赠科研通 3271042
什么是DOI,文献DOI怎么找? 1805207
邀请新用户注册赠送积分活动 882302
科研通“疑难数据库(出版商)”最低求助积分说明 809234