Ancillary Reagents for IHC

Although primary and secondary antibodiesAntibodies secondary are essential for IHC procedure, they cannot be used alone and require ancillary reagentsAncillary reagents : buffer solutions, antibody diluentsAntibody diluent , and mounting mediaMounting media . Without them, IHC staining cannot be successfully started and completed. Let us go over each of them and discuss their role in IHC procedure.