显微镜
超分辨率
薄层荧光显微镜
细菌细胞结构
超分辨显微术
显微镜
样品制备
分辨率(逻辑)
样品(材料)
细菌
纳米技术
材料科学
计算机视觉
荧光显微镜
荧光
活体细胞成像
光学
计算机科学
光学显微镜
人工智能
荧光寿命成像显微镜
细胞
化学
扫描共焦电子显微镜
生物
物理
扫描电子显微镜
图像(数学)
色谱法
生物化学
遗传学
作者
Amy L. Bottomley,Lynne Turnbull,Cynthia B. Whitchurch,Elizabeth J. Harry
出处
期刊:Methods in molecular biology
日期:2016-12-03
卷期号:: 197-209
被引量:7
标识
DOI:10.1007/978-1-4939-6673-8_12
摘要
Advancements in optical microscopy technology have allowed huge progression in the ability to understand protein structure and dynamics in live bacterial cells using fluorescence microscopy. Paramount to high-quality microscopy is good sample preparation to avoid bacterial cell movement that can result in motion blur during image acquisition. Here, we describe two techniques of sample preparation that reduce unwanted cell movement and are suitable for application to a number of bacterial species and imaging methods.
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