成熟
磷酸烯醇丙酮酸羧激酶
磷酸烯醇式丙酮酸羧化酶
李子
生物化学
糖异生
杏
生物
苹果酸酶
苹果酸
柠檬酸合酶
基质(水族馆)
肉体
新陈代谢
ATP柠檬酸裂解酶
酶
柠檬酸
植物
食品科学
栽培
脱氢酶
生态学
作者
Franco Famiani,Daniela Farinelli,Stefano Moscatello,Alberto Battistelli,Richard C. Leegood,Robert P. Walker
标识
DOI:10.1016/j.plaphy.2016.01.007
摘要
The first aim of this study was to determine the contribution of stored malate and citrate to the substrate requirements of metabolism in the ripening flesh of the peach (Prunus persica L. Batsch) cultivar Adriatica. In the flesh, stored malate accumulated before ripening could contribute little or nothing to the net substrate requirements of metabolism. This was because there was synthesis and not dissimilation of malate throughout ripening. Stored citrate could potentially contribute a very small amount (about 5.8%) of the substrate required by metabolism when the whole ripening period was considered, and a maximum of about 7.5% over the latter part of ripening. The second aim of this study was to investigate why phosphoenolpyruvate carboxykinase (PEPCK) an enzyme utilised in gluconeogenesis from malate and citrate is present in peach flesh. The occurrence and localisation of enzymes utilised in the metabolism of malate, citrate and amino acids were determined in peach flesh throughout its development. Phosphoenolpyruvate carboxylase (essential for the synthesis of malate and citrate) was present in the same cells and at the same time as PEPCK and NADP-malic enzyme (both utilised in the dissimilation of malate and citrate). A hypothesis is presented to explain the presence of these enzymes and to account for the likely occurrence of gluconeogenesis.
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