萜类
大肠杆菌
生物化学
生物合成
化学
生产过剩
基因
作者
Parayil Kumaran Ajikumar,Wenhai Xiao,Keith E. J. Tyo,Yong Wang,Fritz Simeon,Effendi Leonard,Oliver Mucha,Too Heng Phon,Blaine A. Pfeifer,Gregory Stephanopoulos
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2010-09-30
卷期号:330 (6000): 70-74
被引量:1445
标识
DOI:10.1126/science.1191652
摘要
Toward Microbial Taxol Taxol, a minor chemical constituent of yew tree bark, has provided a potent cancer treatment. Production methods presently rely on plant cell cultures. Ajikumar et al. (p. 70 ; see the Perspective by Liu and Khosla ) engineered Escherichia coli cells to produce a key taxol precursor, in which the polycyclic carbon skeleton is intact. The approach relied on optimizing the relative activity of two pathways, the first of which synthesized isoprenoid building blocks that were then stitched together with the second pathway. Accumulation of indole as a by-product inhibited the isoprenoid pathway—an insight that should facilitate more efficient engineered biosynthesis of a wide range of commercially important isoprenoid derivatives.
科研通智能强力驱动
Strongly Powered by AbleSci AI