Electrochemical-based ‘‘antibiotsensor’’ for the whole-cell detection of the vancomycin-susceptible bacteria

化学 介电谱 傅里叶变换红外光谱 分析化学(期刊) 生物传感器 循环伏安法 万古霉素 自组装单层膜 检出限 电极 分子 细菌 核化学 电化学 色谱法 化学工程 金黄色葡萄球菌 有机化学 生物化学 物理化学 工程类 遗传学 生物
作者
Araz Norouz Dizaji,Zahraa Hussein Ali,Hamed Ghorbanpoor,Yasin Öztürk,İremnur Akçakoca,Hüseyin Avcı,Fatma Doğan Güzel
出处
期刊:Talanta [Elsevier BV]
卷期号:234: 122695-122695 被引量:22
标识
DOI:10.1016/j.talanta.2021.122695
摘要

In this study, we aim to develop an antibiotic-based biosensor platform 'Antibiotsensor' for the specific detection of gram-positive bacteria using vancomycin modified Screen Printed Gold Electrodes (SPGEs). Through this pathway, vancomycin molecules were first functionalized with thiol groups and characterized with quadrupole time of flight (q-TOF) mass spectroscopy analysis. Immobilization of thiolated vancomycin molecules (HS-Van) onto SPGEs was carried out based on self-assembled monolayer (SAM) phenomenon. Electrochemical impedance spectroscopy (EIS) was employed to test the detection and showed a considerable change in impedance value upon the binding of HS-Van molecules onto the electrode surface. Atomic Force Microscopy analysis indicated that SPGE was successfully modified upon the treatment with HS-Van molecules based on the shift in surface roughness from 173 ± 2 nm to 301 ± 3 nm. Fourier Transform Infrared Spectroscopy (FTIR) spectroscopy proved the EIS and AFM results as well by showing characteristic peaks of immobilized HS-Van molecule. As a proof-of-concept, EIS-based susceptibility testing was performed using Escherichia coli, Staphylococcus aureus and Mycobacterium smegmatis bacteria to prove the specificity of obtained SPGE-Van. EIS data showed that the charge transfer resistance (Rct) values changed from 1.08, 1.18 to 26.5, respectively, indicating that vancomycin susceptible S. aureus was successfully attached onto SPGE-Van surface strongly, while vancomycin resistance E. coli and M. smegmatis did not show any significant attachment properties. In addition, different concentration (108-10 CFU/mL) of S. aureus was performed to investigate sensitivity of proposed sensor platform. Limit of detection and limit of quantitation was calculated as 101.58 and 104.81 CFU/mL, respectively. Scanning electron microscopy (SEM) analysis also confirmed that only S. aureus bacteria was attached to the surface in a dense monolayer distribution. We believe that the proposed approach is selective and sensitive towards the whole-cell detection of vancomycin-susceptible bacteria and can be modified for different purposes in the future.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
入门的橙橙完成签到 ,获得积分10
1秒前
尔尔发布了新的文献求助30
1秒前
红叶完成签到,获得积分10
1秒前
champagnefeng完成签到,获得积分10
3秒前
4秒前
潇洒愚志完成签到,获得积分20
4秒前
杉杉完成签到,获得积分20
5秒前
5秒前
翎儿响叮当完成签到 ,获得积分10
6秒前
pcr163应助小王爱吃饭采纳,获得50
6秒前
纳斯达克发布了新的文献求助10
7秒前
内向姿完成签到,获得积分10
7秒前
量子星尘发布了新的文献求助10
7秒前
追寻的听白完成签到,获得积分10
7秒前
袁睿韬发布了新的文献求助10
7秒前
斯文败类应助lc采纳,获得10
8秒前
Zengyuan完成签到,获得积分10
8秒前
8秒前
9秒前
mokLee63完成签到 ,获得积分10
10秒前
10秒前
10秒前
11秒前
liuxiaoer发布了新的文献求助10
11秒前
拉拉完成签到,获得积分10
12秒前
major发布了新的文献求助10
12秒前
23582发布了新的文献求助10
12秒前
yingzi发布了新的文献求助10
13秒前
害羞的凝竹完成签到 ,获得积分10
13秒前
张玉完成签到,获得积分10
13秒前
开心易真完成签到,获得积分20
15秒前
领导范儿应助有魅力老头采纳,获得10
15秒前
Archer发布了新的文献求助10
15秒前
16秒前
16秒前
16秒前
17秒前
18秒前
活泼的白开水完成签到,获得积分10
19秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
Toward a Combinatorial Approach for the Prediction of IgG Half-Life and Clearance 500
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Picture Books with Same-sex Parented Families: Unintentional Censorship 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3969940
求助须知:如何正确求助?哪些是违规求助? 3514642
关于积分的说明 11175298
捐赠科研通 3249947
什么是DOI,文献DOI怎么找? 1795178
邀请新用户注册赠送积分活动 875617
科研通“疑难数据库(出版商)”最低求助积分说明 804891