Gender selection of offspring has considerable benefit for livestock management systems. Utilization of gender selected semen allows production of male and female offspring to take advantage of sex-limited and sex-influenced traits. Flow cytometric examination of the DNA content of X and Y chromosome-bearing sperm of cattle indicated a difference in their DNA content. Advances in sex-sorting sperm have enabled its incorporation into commercial reproductive management. Several studies have reported in vivo embryo production from superovulated cows and heifers following insemination with sex-sorted sperm. In vitro fertlization requires low numbers of sperm for embryo production. Incidence of stillbirth was similar between heifers inseminated with conventional or sex-sorted semen. The fertility of sex-sorted semen in cattle has always been lower than that of conventional semen. The SexedULTRA™ process was devised as a system to simplify and optimize the media used for the sex-sorting protocol.