下调和上调
细胞生长
癌症研究
癌症
细胞凋亡
细胞周期检查点
药理学
细胞周期
化学
生物
医学
生物化学
内科学
基因
作者
Danfeng Wang,Yuqing Zhao,Yimeng Wang,Rong Yan,Hongshuang Qin,Yongli Bao,Zhenbo Song,Chunlei Yu,Luguo Sun,Yuxin Li
出处
期刊:Tumor Biology
[SAGE]
日期:2016-04-02
卷期号:37 (9): 11805-11813
被引量:7
标识
DOI:10.1007/s13277-016-5037-7
摘要
While the incidence of cancer continues to increase, the current therapeutic options remain imperfect. Therefore, there is an urgent need to discover new targeted anti-cancer therapies. Testes-specific protease 50 (TSP50) is abnormally expressed in most cancer tissues and downregulation of TSP50 expression can reduce cell proliferation and induce cell apoptosis, which makes it a potential target for cancer therapy. In this study, we constructed a firefly luciferase reporter pGL3-TSP50-3′-UTR as a drug screening model to screen potential candidate compounds that target TSP50 mRNA. We identified the compound 7P3A, which consists of 70 % 25-methoxyl-dammarane-3β, 12β, 20-triol and 30 % artemisinin, as being capable of inhibiting the TSP50-3′-UTR reporter activity, as well as the expression of TSP50. Further investigation revealed that 7P3A could inhibit MDA-MB-231 cell proliferation and induce cell cycle arrest, and over-expression of TSP50 partially reversed the effect of 7P3A. In vivo investigation showed that 7P3A could inhibit tumor growth in a xenograft model of breast cancer. These results suggest that 7P3A exhibits anti-cancer effects, in part, through downregulation of TSP50 expression.
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