褐藻糖胶
化学
岩藻糖
色谱法
链酶
硫酸盐
部分凝血活酶时间
分数(化学)
半乳糖
食品科学
多糖
植物
生物化学
生物
有机化学
胰蛋白酶
血小板
免疫学
酶
作者
Jae-Geun Koo,Choi Yong-Seok,Kwak Jung-Ki
出处
期刊:Korean Journal of Fisheries and Aquatic Sciences
[The Korean Society of Fisheries and Aquatic Science]
日期:2001-01-01
卷期号:34 (5): 515-520
被引量:24
摘要
The anticoagulant activities of fucoidan fractions extracted from Sporophylls of Undaria pinnatifida, Laminaria religiosa, Hizikia fusiforme and Sargassum fulvellum were studied to assess the relationship between chemical characteristics and the activities. Crude fucoidans extracted with diluted HCl solution (pH 2.0) at were precipitated with cetylpyridinum chloride and then fractionated by dissolving the precipitated complex with increasing , concentrations (1.0 M, 1.5 M, 3.0 M). The anticoagulant activities of the fractions with respect to activated partial thromboplastin (APTT) increased with increase in their sulfate content and Undaria finnatifida Fr-3.0 fraction, prepared by dissolving with 3.0 M solution, exhibited the highest activity. The Undaria finnatifida Fr-3.0 fraction was further modified with pronase and laminase. The pronase and laminase treatment decreased protein and glucose content and the APTT activity was higher than that or parent Undaria finnatifida Fr-3.0 fraction. The pronase and laminase modified Undaria finnatifida Fr-3.0 was composed of fucose, galactose, mannose, sulfate, uronic acid in the approximately molar ratio of 1.00 : 1.30: 0.03 : 2.70 : 0.08.
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