Development of a LC-MS/MS method to measure serum 3-sulfate and 3-glucuronide 25-hydroxyvitamin D3 metabolites; comparisons to unconjugated 25OHD in pregnancy and polycystic ovary syndrome

化学 色谱法
作者
Kevin Huynh,Punith Kempegowda,Jennifer Tamblyn,Maeve Reilly,Jon Wolf Mueller,Martin Hewison,Carl Jenkinson
出处
期刊:Steroids [Elsevier BV]
卷期号:169: 108812-108812 被引量:9
标识
DOI:10.1016/j.steroids.2021.108812
摘要

Vitamin D status is routinely assessed by measuring circulating concentrations of 25-hydroxyvitamin D (25OHD2 or 25OHD3). However as deconjugation is not routinely incorporated into sample treatment prior to analysis, conjugated forms of 25OHD (particularly the more abundant 25OHD3) are often not considered in determining serum concentrations of total 25OHD. Two major circulating conjugated forms of 25OHD3 are 25-hydroxyvitamin D3-3-sulfate (25OHD3-S) and 25-hydroxyvitamin D3-3-glucuronide (25OHD3-G). Incorporating these two conjugated metabolites into the measurement of vitamin D status could improve our understanding of vitamin D status in health, particularly if there are changes in sulfation and glucuronidation activities. The aim of this study was to develop a liquid chromatography tandem-mass spectrometry (LC-MS/MS) targeted method for measurement of 25OHD3-S and 25OHD3-G in serum to enable comparisons with circulating levels of the free 25OHD3 form. We developed and validated a new LC-MS/MS method that measured both 25OHD3-S and 25OHD3-G following a solid phase extraction sample preparation method. Partial separation of analytes by LC, and the separation of analytes by the optimized multiple reaction monitoring transitions enabled the quantitation of both 25OHD3-S and 25OHD3-G in the single method. Serum concentrations of 25OHD3-S (24.7 ± 11.8 ng/mL) and 25OHD3-G (2.4 ± 1.2 ng/mL) were shown to be a significant proportion of circulating vitamin D metabolites in healthy donor serums. These levels of 25OHD3-S and 25OHD3-G closely associated with 25OHD3 concentrations, r = 0.728, p = 0.001 and r = 0.632, p = 0.006 respectively. However in serum from pregnant women and non-pregnant women with polycystic ovary syndrome (PCOS) significant differences in the ratios between conjugated and free 25OHD3 were observed between pregnancy groups (25OHD3/25OHD3-S and 25OHD3/25OHD3-G p < 0.001), and between healthy and PCOS subjects (25OHD3/25OHD3-G p < 0.050). Development of this novel high-throughput LC-MS/MS method indicates that 25OHD3-S and 25OHD3-G are substantial components of circulating vitamin D metabolites. The concentrations of these metabolites relative to conventional 25OHD3 may vary in different physiological and pathophysiological settings, and may therefore play an unrecognized but important role in the actions of vitamin D.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
清浅完成签到,获得积分10
刚刚
王冉冉发布了新的文献求助10
1秒前
2秒前
所所应助zjz采纳,获得30
3秒前
3秒前
小吴同学发布了新的文献求助10
4秒前
量子星尘发布了新的文献求助10
4秒前
4秒前
5秒前
shaocat完成签到 ,获得积分10
5秒前
风中的眼神完成签到,获得积分10
5秒前
CAOHOU应助奋斗水香采纳,获得10
6秒前
俊逸梦蕊完成签到,获得积分10
6秒前
7秒前
典雅牛青关注了科研通微信公众号
7秒前
Xinxxx发布了新的文献求助10
7秒前
illusion完成签到,获得积分10
8秒前
wanci应助王冉冉采纳,获得30
9秒前
树小夏发布了新的文献求助10
10秒前
小吴同学完成签到,获得积分10
10秒前
赘婿应助kk_yang采纳,获得10
11秒前
成就伟祺关注了科研通微信公众号
12秒前
能干的语芙完成签到 ,获得积分10
12秒前
无欲无求傻傻完成签到,获得积分10
12秒前
12秒前
12秒前
尊敬寒松完成签到 ,获得积分10
12秒前
糊涂的麦片完成签到,获得积分10
12秒前
13秒前
13秒前
14秒前
wanci应助wangdafa采纳,获得10
14秒前
竹子co完成签到,获得积分10
14秒前
steventj完成签到,获得积分10
14秒前
yz完成签到 ,获得积分10
15秒前
朴实山兰完成签到,获得积分10
16秒前
tkkdy发布了新的文献求助10
16秒前
蓁蓁发布了新的文献求助10
16秒前
醉熏的鑫发布了新的文献求助10
17秒前
独家双层汉堡完成签到,获得积分10
17秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds第二卷 1200
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038657
求助须知:如何正确求助?哪些是违规求助? 3576306
关于积分的说明 11375198
捐赠科研通 3306108
什么是DOI,文献DOI怎么找? 1819379
邀请新用户注册赠送积分活动 892698
科研通“疑难数据库(出版商)”最低求助积分说明 815066