厌氧氨氧化菌
浮霉菌门
酸杆菌
细菌
氯仿(类)
图书馆
生物
16S核糖体RNA
土壤水分
疣状疣
土壤微生物学
氮气循环
水田
微生物种群生物学
厚壁菌
生态学
化学
氮气
反硝化
遗传学
有机化学
反硝化细菌
作者
Anamika Khanal,Seul Lee,Jihoon Lee
出处
期刊:Korean Journal of Environmental Agriculture
[The Korean Society of Environmental Agriculture]
日期:2020-03-31
标识
DOI:10.5338/kjea.2020.39.1.4
摘要
BACKGROUND: Microbes that govern a unique biochemical process of oxidizing ammonia into dinitrogen gas, such as anaerobic ammonium oxidation (anammox) have been reported to play a pivotal role in agricultural soils and in oceanic environments. However, limited information for anammox bacterial abundance and distribution in the terrestrial habitats has been known.
METHODS AND RESULTS: Phylogenetic and next-generation sequencing analyses of bacterial 16S rRNA gene were performed to examine potential anammox bacteria in paddy soils. Through clone libraries constructed by using the anammox bacteria-specific primers, some clones showed sequence similarities with Planctomycetes (87% to 99%) and anammox bacteria (94% to 95%). Microbial community analysis for the paddy soils by using Illumina Miseq sequencing of 16S rRNA gene at phylum level was dominated by unclassified Bacteria at 33.2 ± 7.6%, followed by Chloroflexi at 20.4 ± 2.0% and Acidobacteria at 17.0 ± 6.5%. Planctomycetes that anammox bacteria are belonged to was 1.5% (± 0.3) on average from the two paddy soils.
CONCLUSION: We suggest evidence of anammox bacteria in the paddy soil. In addition to the relatively well-known microbial processes for nitrogen-cycle, anammox can be a potential contributor on the cycle in terrestrial environments such as paddy soils.
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