Abstract Three new aromatic rutinosides, including 2-methoxy-4-methylphenyl 1-O-rutinoside (1), 3,5-dimethoxy-4-hydroxyphenyl 1-O-rutinoside (3) and (S)-2-hydroxy-2-phenylethyl 1-O-rutinoside (6) were isolated from the fresh aerial roots of Pandanus tectorius. Additionally, the 12 known compounds were identified; 2-methoxy-4-hydroxymethylphenyl 1-O-rutinoside, cuneataside D, benzyl rutinoside, 2-phenethyl rutinoside, viridoside, 7,8-threo-7-methoxysyringylglycerol, 7,8-erythro-7-methoxysyringylglycerol, methyl caffeate, 3,5-di-O-caffeoyl-quinic acid 1-methyl ether, 4,5-di-O-caffeoyl-quinic acid 1-methyl ether, 3,4-di-O-caffeoyl-epi-quinic acid methyl ester and hedyotol D 7-O-β-D-glucopyranoside. The structure determinations were considered based on the physical data and the spectroscopic evidence including 1D and 2D NMR experiments.