荧光
粘度
溶酶体
化学
溶血酶-
甘油
生物物理学
分析化学(期刊)
材料科学
色谱法
生物化学
光学
探测器
物理
复合材料
闪烁体
酶
生物
作者
Defang Han,Jundan Yi,L.K. Chang,Lijuan Liang,Kun Huang,Lin‐Hai Jing,Da‐Bin Qin
标识
DOI:10.1016/j.saa.2020.118405
摘要
A new fluorescent probe Lyso-Fl has been facilely prepared by an esterification reaction of spironolactone fluoran dye Rdi with ethanol, which shows viscosity-selective response by fluorescence. The new probe delivers obvious fluorescence signal enhancement when environmental viscosity changes from 1.01 cP (water) to 1256 cP (98% glycerol). And, both the emission intensity (575 nm) and fluorescence lifetime of Lyso-Fl exhibit individually good linear relationships with the solution viscosity. Besides, Lyso-Fl gives a selective response to viscosity among various biological species and exhibits pH-independent (1–10) fluorescent signals towards viscosity. More importantly, Lyso-Fl shows low cytotoxicity and can be utilized for monitoring of dexamethasone-stimulated viscosity enhancement by cell imaging with excellent lysosome-targeted performance, promoting it a promising fluorescent probe for lysosomal viscosity detection.
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