Systematic Quantification of Neurotrophic Adipokines RBP4, PEDF, and Clusterin in Human Cerebrospinal Fluid and Serum

化学
作者
Alexandra Höpfinger,Martin Berghoff,Thomas Karrasch,Andreas Schmid,Andreas Schäffler
出处
期刊:The Journal of Clinical Endocrinology and Metabolism [Oxford University Press]
卷期号:106 (5) 被引量:3
标识
DOI:10.1210/clinem/dgaa983
摘要

Context Data on the presence/quantification of the neurotrophic adipokines retinol-binding protein-4 (RBP4), clusterin, and pigment epithelium-derived factor (PEDF) in human cerebrospinal fluid (CSF) are scarce and migration of these adipokines across of the blood-brain barrier (BBB) is uncertain. Objective This work aimed to quantify RBP4, PEDF, and clusterin in paired serum and CSF samples of patients undergoing neurological evaluation. Methods A total of 268 patients (109 male, 159 female) were included. Adipokine serum and CSF concentrations were measured by enzyme-linked immunosorbent assay in duplicate. Results RBP4 was abundant in serum (mean, 31.9 ± 24.2 μg/mL). The serum concentrations were approximately 145 times higher than in CSF (CSF to serum RBP4 ratio, 8.2 ± 4.3 × 10-3). PEDF was detectable in serum (mean, 30.2 ± 11.7 μg/mL) and concentrations were approximately 25 times higher than in CSF (CSF to serum PEDF ratio, 42.3 ± 15.6 × 10-3). Clusterin serum concentrations were abundant with mean levels of 346.0 ± 114.6 μg/mL, which were approximately 40 times higher than CSF levels (CSF to serum clusterin ratio, 29.6 ± 23.4 × 10-3). RBP4 and PEDF serum levels correlated positively with CSF levels, which were increased in overweight/obese patients and in type 2 diabetic patients. The CSF concentrations of all 3 adipokines increased with BBB dysfunction. RBP4 in CSF correlated positively with inflammatory parameters. In detail, only RBP4 showed the kinetics and associations that are mandatory for a putative mediator of the fat-brain axis. Conclusion RBP4, PEDF, and clusterin are permeable to the BBB and increase with the measure of BBB dysfunction. RBP4 represents an inflammatory neurotrophic adipokine and is a promising mediator of the fat-brain axis.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
romeo发布了新的文献求助10
1秒前
妖孽宇完成签到,获得积分10
3秒前
简简单单完成签到,获得积分10
3秒前
550190946发布了新的文献求助10
3秒前
5秒前
111完成签到,获得积分10
5秒前
zhubin完成签到 ,获得积分10
5秒前
7秒前
田南松发布了新的文献求助10
10秒前
搬砖美少女完成签到,获得积分10
10秒前
nn发布了新的文献求助10
11秒前
7ohnny完成签到,获得积分10
12秒前
apckkk完成签到 ,获得积分10
14秒前
深情安青应助550190946采纳,获得10
15秒前
16秒前
17秒前
jbq完成签到 ,获得积分20
17秒前
YM完成签到,获得积分10
19秒前
生动柔发布了新的文献求助10
19秒前
大旭完成签到 ,获得积分10
20秒前
Fn完成签到 ,获得积分10
22秒前
zero完成签到,获得积分10
24秒前
瘦瘦谷兰完成签到,获得积分10
24秒前
zcz完成签到 ,获得积分10
25秒前
白嘉乐完成签到,获得积分10
26秒前
考研小白完成签到,获得积分10
26秒前
高妍纯完成签到 ,获得积分10
28秒前
30秒前
风中的丝袜完成签到,获得积分10
30秒前
赵赵完成签到,获得积分10
33秒前
33秒前
shiizii应助科研通管家采纳,获得10
34秒前
xuzj应助科研通管家采纳,获得10
34秒前
Mars应助科研通管家采纳,获得30
34秒前
Hello应助科研通管家采纳,获得10
34秒前
叶梓轩完成签到 ,获得积分10
34秒前
上官若男应助科研通管家采纳,获得10
34秒前
34秒前
34秒前
机智的锦程完成签到 ,获得积分10
35秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038201
求助须知:如何正确求助?哪些是违规求助? 3575940
关于积分的说明 11373987
捐赠科研通 3305747
什么是DOI,文献DOI怎么找? 1819274
邀请新用户注册赠送积分活动 892662
科研通“疑难数据库(出版商)”最低求助积分说明 815022