Contribution of Glutathione S-Transferases to Imidacloprid Resistance in Nilaparvata lugens

益达胺 谷胱甘肽 谷胱甘肽转移酶 化学 抗药性 生物 毒理 生物化学 杀虫剂 农学
作者
Bob Yang,Xiangze Lin,Na Yu,Haoli Gao,Yixi Zhang,Wei Liu,Zewen Liu
出处
期刊:Journal of Agricultural and Food Chemistry [American Chemical Society]
卷期号:68 (52): 15403-15408 被引量:28
标识
DOI:10.1021/acs.jafc.0c05763
摘要

The importance of glutathione S-transferases (GSTs) in imidacloprid resistance in Nilaparvata lugens, a major rice pest, and other insects was often excluded, mostly due to the slight effects of diethyl maleate (DEM) on synergizing imidacloprid in resistant populations. Here, we found that the synergistic effects of DEM were time-dependent. At 24 or 48 h, the time often selected to record mortalities in imidacloprid bioassay, DEM really did not cause an obvious increase in imidacloprid toxicity. However, significant effects were observed after 72 h. The results revealed that GSTs, as phase II detoxification enzymes to metabolize secondary products generated from phase I detoxification enzymes, were also important in imidacloprid resistance in N. lugens, but might have occurred a little later than that of P450s and CarEs as phase I enzymes. The constitutive overexpression in the imidacloprid-resistant strain G25 and expression induction by imidacloprid in the susceptible strain S25 indicated that four GST genes, NlGSTs1, NlGSTs2, NlGSTe1, and NlGSTm1, were important in imidacloprid resistance, which was confirmed by RNAi test. The higher expression levels and more expression induction by imidacloprid in the midgut and fat body compared to the whole insect supported the important roles of these four GSTs, which was also supported by the more overexpression times in the midgut and fat body versus the whole insect between G25 and S25 strains. Taking the data together, the study ascertained the roles of GSTs in imidacloprid resistance in N. lugens.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
sks关注了科研通微信公众号
2秒前
汉堡包应助光芒万丈采纳,获得10
4秒前
科研发布了新的文献求助100
5秒前
showitt发布了新的文献求助10
6秒前
Z_Z完成签到,获得积分10
7秒前
8秒前
KYTZL发布了新的文献求助10
8秒前
zhanks发布了新的文献求助10
11秒前
爆米花应助科研通管家采纳,获得10
13秒前
SciGPT应助科研通管家采纳,获得10
13秒前
13秒前
13秒前
科研通AI2S应助科研通管家采纳,获得10
14秒前
852应助科研通管家采纳,获得10
14秒前
Owen应助中中采纳,获得10
14秒前
14秒前
14秒前
14秒前
15秒前
18秒前
echo完成签到,获得积分10
20秒前
KYTZL完成签到,获得积分10
21秒前
CCC发布了新的文献求助10
22秒前
许峰完成签到,获得积分10
23秒前
23秒前
24秒前
犹豫溪灵完成签到,获得积分10
26秒前
28秒前
中中发布了新的文献求助10
30秒前
空白幻想丶完成签到,获得积分10
30秒前
xzy998应助张朝程采纳,获得10
30秒前
犹豫溪灵发布了新的文献求助10
32秒前
香蕉饼干完成签到,获得积分10
32秒前
前寒武完成签到,获得积分10
32秒前
34秒前
壹号完成签到,获得积分10
34秒前
潇潇暮雨发布了新的文献求助20
35秒前
36秒前
陈权完成签到 ,获得积分10
36秒前
高分求助中
Sustainability in Tides Chemistry 1500
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
CLSI EP47 Evaluation of Reagent Carryover Effects on Test Results, 1st Edition 800
Threaded Harmony: A Sustainable Approach to Fashion 799
Livre et militantisme : La Cité éditeur 1958-1967 500
Retention of title in secured transactions law from a creditor's perspective: A comparative analysis of selected (non-)functional approaches 500
"Sixth plenary session of the Eighth Central Committee of the Communist Party of China" 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3055401
求助须知:如何正确求助?哪些是违规求助? 2712227
关于积分的说明 7430195
捐赠科研通 2357037
什么是DOI,文献DOI怎么找? 1248528
科研通“疑难数据库(出版商)”最低求助积分说明 606737
版权声明 596093