外体
化学
适体
微泡
尿
蛋白质组
分离(微生物学)
DNA
CD63
生物化学
分子生物学
小RNA
生物信息学
生物
基因
作者
Ning Zhang,Nianrong Sun,Chunhui Deng
出处
期刊:Talanta
[Elsevier]
日期:2020-09-01
卷期号:221: 121571-121571
被引量:58
标识
DOI:10.1016/j.talanta.2020.121571
摘要
There are accumulating evidence that proteins carried by exosomes in urine are most possibly used as biomarkers or therapeutic carriers for certain diseases. The isolation of exosomes is therefore highly desirable for aiding the downstream protein analysis. Particularly, urine is a dynamic biological fluid changing within a short time, resulting in that the separation of urinary exosome requires more efficient technology. Here, a new biocompatible material (denoted as Fe3O4@TiO2-CD63 aptamer) is designed and synthesized for rapid exosome isolation from human urine, depending on the double interactions of TiO2 with phosphate groups as well as aptamers with specific exosome proteins. Moreover, within 10 min, 92.6% exosomes with intact structure are captured from urine by Fe3O4@TiO2-CD63 aptamers, from which 999 proteins are detected through LC-MS/MS.
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