断点
染色体易位
互惠的
计算生物学
分辨率(逻辑)
生物
计算机科学
遗传学
人工智能
基因
语言学
哲学
作者
Judy F.C. Chow,Heidi H. Y. Cheng,Esther Yuet Ying Lau,William S.B. Yeung,Ernest H.Y. Ng
出处
期刊:MethodsX
[Elsevier]
日期:2019-01-01
卷期号:6: 2499-2503
被引量:6
标识
DOI:10.1016/j.mex.2019.10.028
摘要
Long-read nanopore sequencing enables direct high-resolution breakpoint mapping on balanced carriers of reciprocal translocation. The mean sequencing depth on the translocated chromosomes to achieve accurate mapping of breakpoints ranged from 2.5-fold to 6.2-fold. To speed up determination of the breakpoints from long-read sequencing data, alignment reads on the translocated chromosomes were extracted before piped into NanoSV. Checking the position of breakpoints on Interactive Genomics Viewer (IGV) was crucial to successful design of breakpoint PCR primers, especially when large deletion was involved at the breakpoints. •Long-read sequencing enables accurate breakpoint mapping with base-pair resolution•Splitting bam files by translocated chromosomes drastically speeded up the breakpoint determination•IGV helps to identify the breakpoint positions and facilitate the design of breakpoint PCR primers.
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