Formation of inclusion complexes of starch with different organic compounds. III, Study of ligand binding in binary model systems with (-)limonene

The formation of inclusion complexes between starch and (−) limonene was studied in binary model systems. Binding equilibria approximated by the Hill equation, were analysed using the affinity spectrum method. Binding parameters were estimated by means of regression methods. Linearized Scatchard plot was used as a criterion of the fit. For evaluation of binding profiles and binding parameters a sigmoid continuation of the isotherm was constructed. Possible inhibitory effects are related to the hydrophobic and non-polar nature of (−)limonene