卵黄囊
生物
细胞命运测定
胚胎
髓样
祖细胞
细胞生物学
命运图
核糖核酸
计算生物学
干细胞
免疫学
遗传学
基因
转录因子
作者
Yulai Zhao,Juan Song,Xuewei Bao,J.L. Zhang,Joseph C. Wu,Lu‐Yang Wang,Caroline He,Wei Shao,X L Bai,Qi Zhang,Jin-Huan Sheng
出处
期刊:Cell Reports
[Elsevier]
日期:2023-11-01
卷期号:42 (11): 113364-113364
被引量:3
标识
DOI:10.1016/j.celrep.2023.113364
摘要
Erythro-myeloid progenitors of the yolk sac that originates during early embryo development has been suggested to generate tissue-resident macrophage, mast cell, and even endothelial cell populations from fetal to adult stages. However, the heterogeneity of erythro-myeloid progenitors (EMPs) is not well characterized. Here, we adapt single-cell RNA sequencing to dissect the heterogeneity of EMPs and establish several fate-mapping tools for each EMP subset to trace the contributions of different EMP subsets. We identify two primitive and one definitive EMP subsets from the yolk sac. In addition, we find that primitive EMPs are decoupled from definitive EMPs. Furthermore, we confirm that primitive and definitive EMPs give rise to microglia and other tissue-resident macrophages, respectively. In contrast, only Kit+ Csf1r− primitive EMPs generate endothelial cells transiently during early embryo development. Overall, our results delineate the contribution of yolk sac EMPs more clearly based on the single-cell RNA sequencing (scRNA-seq)-guided fate-mapping toolkit.
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