拟南芥
热冲击系数
拟南芥
下胚轴
突变体
生物
细胞生物学
热休克蛋白
转录组
热冲击
基因
基因表达
植物
遗传学
热休克蛋白70
作者
Bingjie Li,Shimeng Jiang,Liang Gao,Wenhui Wang,H R Luo,Yining Dong,Zhihua Gao,Shuzhi Zheng,Xinye Liu,Wenqiang Tang
摘要
ABSTRACT Thermomorphogenesis and the heat shock (HS) response are distinct thermal responses in plants that are regulated by PHYTOCHROME‐INTERACTING FACTOR 4 (PIF4) and HEAT SHOCK FACTOR A1s (HSFA1s), respectively. Little is known about whether these responses are interconnected and whether they are activated by similar mechanisms. An analysis of transcriptome dynamics in response to warm temperature (28°C) treatment revealed that 30 min of exposure activated the expression of a subset of HSFA1 target genes in Arabidopsis thaliana . Meanwhile, a loss‐of‐function HSFA1 quadruple mutant ( hsfa1‐cq ) was insensitive to warm temperature‐induced hypocotyl growth. In hsfa1‐cq plants grown at 28°C, the protein and transcript levels of PIF4 were greatly reduced, and the circadian rhythm of many thermomorphogenesis‐related genes (including PIF4 ) was disturbed. Additionally, the nuclear localization of HSFA1s and the binding of HSFA1d to the PIF4 promoter increased following warm temperature exposure, whereas PIF4 overexpression in hsfa1‐cq partially rescued the altered warm temperature‐induced hypocotyl growth of the mutant. Taken together, these results suggest that HSFA1s are required for PIF4 accumulation at a warm temperature, and they establish a central role for HSFA1s in regulating both thermomorphogenesis and HS responses in Arabidopsis.
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