计算生物学
清脆的
阿尔戈瑙特
核酸检测
核酸
注意事项
严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)
检测点注意事项
分子诊断学
生物
2019年冠状病毒病(COVID-19)
核糖核酸
生物信息学
医学
疾病
遗传学
RNA干扰
传染病(医学专业)
护理部
病理
免疫学
基因
作者
Kai Sun,Yan Liu,Wei Zhao,Biao Ma,Mingzhou Zhang,Xiaoping Yu,Zihong Ye
标识
DOI:10.3390/ijms241914987
摘要
The recent pandemic of SARS-CoV-2 has underscored the critical need for rapid and precise viral detection technologies. Point-of-care (POC) technologies, which offer immediate and accurate testing at or near the site of patient care, have become a cornerstone of modern medicine. Prokaryotic Argonaute proteins (pAgo), proficient in recognizing target RNA or DNA with complementary sequences, have emerged as potential game-changers. pAgo present several advantages over the currently popular CRISPR/Cas systems-based POC diagnostics, including the absence of a PAM sequence requirement, the use of shorter nucleic acid molecules as guides, and a smaller protein size. This review provides a comprehensive overview of pAgo protein detection platforms and critically assesses their potential in the field of viral POC diagnostics. The objective is to catalyze further research and innovation in pAgo nucleic acid detection and diagnostics, ultimately facilitating the creation of enhanced diagnostic tools for clinic viral infections in POC settings.
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