化学
细胞生物学
基因表达
基因
分子生物学
生物
生物化学
作者
Devaprasad Markandeyan,Shiek S. S. J. Ahmed,Palani Perumal,Arulvasu Chinnasamy,Bhavatarini Govindaraj,N. Sekar,M. Sudhan,Priyadarshini Shanmugam,V. Janakiraman,Deepakrajasekar Padmanaban,Perumal Jayaraman
出处
期刊:International journal of experimental research and review
[International Academic Publishing House]
日期:2024-03-30
卷期号:37: 120-130
标识
DOI:10.52756/ijerr.2024.v37spl.010
摘要
This study aimed to investigate the impact of Borreria hispida extract on gene expression in NRK-52E cells under conditions of glucotoxicity. Gene expression analysis was conducted using RT-PCR following the exposure of cultured NRK-52E cells to glucotoxic conditions and varying concentrations of Borreria hispida extract. The results demonstrated a dose-dependent increase in SIRT1 gene expression and a concomitant decrease in HIF-1λ, ET-1, and VEGFR-2 gene expressions upon treatment with Borreria hispida extract. Additionally, molecular docking studies suggested the potential inhibition of Rho-kinase as a mechanistic explanation for these effects. Borreria hispida extract may confer renoprotective benefits against glucotoxicity-induced cellular damage. The potential therapeutic utility of Borreria hispida extracts in managing renal complications associated with conditions such as diabetes. Furthermore, our docking studies shed light on the potential molecular mechanisms underlying the observed effects, suggesting interactions between phytochemicals present in Borreria hispida extract and the JNK-1 protein. These interactions may contribute to the augmentation of SIRT1 activity, further bolstering the extract's therapeutic utility in DKD.
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