Enrichment of Naked Amoebae Species

In spite of various studies on naked amoebae species across the world, the diversity of naked amoebae in various habitats is still poorly studied. The use of appropriate cultivation media and methods is preferred to study the species spectra and ecology in soil and freshwater samples in order to cultivate a broad range of amoebae species. For recovery of the species diversity of naked amoebae, efficiency of cultivation media plays a key role where usually six enrichment media – two liquid and four agar media – are preferred. The enrichment media described here include Prescott-James (PJ), grass-seed infusion (GS), grass-seed agar (GSA), GSA with an overlay of water (GSA + W), non-nutrient agar (NNA), and NNA with an overlay of water (NNA + W). To study the ecology and diversity of naked amoebae from freshwater samples, grass-seed infusions were found to be the most effective media, and its preparation method was also found to be easy.