化学
DPPH
阿布茨
抗氧化剂
酪氨酸酶
食品科学
淀粉酶
香兰素
特罗洛克
色谱法
生物化学
酶
作者
Damira Ongalbek,Özge Tokul-Ölmez,Bihter Şahin,Selçuk Küçükaydın,Fatma Aydoğmuş-Öztürk,Yusuf Sıcak,Balakyz Yeskaliyeva,Mehmet Öztürk
出处
期刊:Food Chemistry
[Elsevier]
日期:2024-09-01
卷期号:451: 139409-139409
被引量:2
标识
DOI:10.1016/j.foodchem.2024.139409
摘要
Herein, nineteen buckwheat honey samples collected from 19 stations of different ecological zones of Kazakhstan were analysed for their pollen density, physicochemical properties, chemical composition, antioxidant, anticholinesterase, tyrosinase inhibitory, and urease inhibitory activities with chemometric approaches. Twelve phenolic compounds and fumaric acid were identified using HPLC-DAD, and mainly fumaric, p-hydroxybenzoic, p-coumaric, trans-2-hydroxy cinnamic acids, and chrysin were detected in all samples. The honey samples collected from the Northern zone exhibited best antioxidant activity in lipid peroxidation inhibitory (IC50:8.65 ± 0.50 mg/mL), DPPH• (IC50:17.07 ± 1.49 mg/mL), ABTS•+ (IC50:8.90 ± 0.65 mg/mL), CUPRAC (A0.50:7.51 ± 0.30 mg/mL) and metal chelating assay (IC50:10.39 ± 0.71 mg/mL). In contrast, South-eastern zone samples indicated better acetylcholinesterase (55.57 ± 0.83%), butyrylcholinesterase (49.59 ± 1.09%), tyrosinase (44.40 ± 1.21%), and moderate urease (24.57 ± 0.33%) inhibitory activities at 20 mg/mL. The chemometric classification of nineteen buckwheat honey was performed using PCA and HCA techniques. Both were supported by correlation analysis. Thirteen compounds contributed significantly to the clustering of buckwheat honey based on geographical origin.
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