Screening and Capability Assessment of Tyrosinase Inhibitors in Isodon excisoides by Ultrafiltration Coupled with UHPLC‐Q‐TOF‐MS and Molecular Docking Technology

酪氨酸酶 化学 色谱法 IC50型 对接(动物) 生物化学 体外 医学 护理部
作者
Xinming Jia,Shilin Sun,Qian Zhang,Nan Wang,Mengxin Yang,Yiran Jin,Yingfeng Du
出处
期刊:Chemistry & Biodiversity [Wiley]
卷期号:19 (12) 被引量:1
标识
DOI:10.1002/cbdv.202200748
摘要

Tyrosinase inhibitors can alleviate the harm to the liver caused by tyrosinase. How to effectively screen out natural tyrosinase inhibitors becomes a focus. In this study, Isodon excisoides was first extracted with the ultrasound optimized by response surface methodology. Then, a method combined ultrafiltration with ultra-liquid chromatography mass spectrometry (UHPLC/MS) was built to screen and identify tyrosinase inhibitors. The binding energies of active ingredients to tyrosinase were calculated by molecular docking. The reliability of the results was validated by the IC50 of enzyme inhibition assay. As a result, the binding energies of 7 components including excisanin B, lasiokaurin, rabdophyllin G, rabdoserrin B, rabdosin D, rabdosinate and weisiensin were lower than that of resveratrol. It was indicated that these components had high tyrosinase inhibitory activity. The IC50 values of lasiokaurin and excisanin B were 177 and 142 μmol/mL, which were less than that of resveratrol (183 μmol/mL). It showed that this way was simple, rapid, reliable and effective, which provided a new strategy to screen natural bioactive compounds from plants.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
今后应助linli采纳,获得10
2秒前
3秒前
今后应助111采纳,获得10
3秒前
ding应助欣慰的乌冬面采纳,获得10
3秒前
ceci完成签到,获得积分10
5秒前
123完成签到,获得积分10
5秒前
今后应助我爱学习采纳,获得10
6秒前
lei029发布了新的文献求助10
6秒前
徐婷完成签到,获得积分10
6秒前
Versa完成签到,获得积分10
8秒前
jindui完成签到,获得积分10
8秒前
9秒前
10秒前
在水一方应助泡泡糖采纳,获得10
10秒前
annie完成签到,获得积分10
11秒前
今后应助科研通管家采纳,获得10
12秒前
yar应助科研通管家采纳,获得10
12秒前
yar应助科研通管家采纳,获得10
12秒前
共享精神应助科研通管家采纳,获得10
12秒前
yar应助科研通管家采纳,获得10
12秒前
所所应助科研通管家采纳,获得10
12秒前
小马甲应助科研通管家采纳,获得10
12秒前
小蘑菇应助科研通管家采纳,获得10
13秒前
李爱国应助科研通管家采纳,获得10
13秒前
yar应助科研通管家采纳,获得10
13秒前
bkagyin应助科研通管家采纳,获得10
13秒前
yar应助科研通管家采纳,获得10
13秒前
yar应助科研通管家采纳,获得10
13秒前
13秒前
15秒前
爱尚完成签到,获得积分10
15秒前
15秒前
LL发布了新的文献求助10
17秒前
ajiaxi完成签到,获得积分10
18秒前
共享精神应助科研眼镜蛇采纳,获得10
20秒前
22秒前
FashionBoy应助才下眉头采纳,获得10
24秒前
量子星尘发布了新的文献求助10
24秒前
Owen应助独特冰安采纳,获得10
28秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3975426
求助须知:如何正确求助?哪些是违规求助? 3519848
关于积分的说明 11199831
捐赠科研通 3256122
什么是DOI,文献DOI怎么找? 1798124
邀请新用户注册赠送积分活动 877386
科研通“疑难数据库(出版商)”最低求助积分说明 806305