RNA干扰
细胞生物学
生物
生物化学
自噬
蘑菇体
转基因
酪氨酸
基因
核糖核酸
细胞凋亡
黑腹果蝇
作者
Yujing Li,Yuxuan Liang,Chunxiu Peng,Jiashun Gong
标识
DOI:10.1016/j.ijbiomac.2024.136476
摘要
Black truffle (Tuber sinense) is a famous luxurious mushroom with abundant protein resources. Nevertheless, until now, no single study has explored the potential function of black truffle protein in any animal models. Thus, this study investigated the sleep-promoting effects of truffle albumin (TA) and its hydrolysate (TAH). Then, two novel sleep-enhancing peptides were explored from TAH. Our results showed that TA and TAH significantly prolonged the total sleep time and improved sleep quality of insomnia Drosophila. Additionally, two novel peptides YLDLAPL and YLRPEGDW with strong sleep-enhancing activity were explored by virtual screening and Drosophila with transgenic RNA interference (RNAi) technology. Finally, the transcriptomics analysis investigated potential mechanisms of sleep-enhancing effects in Drosophila: (1) regulation of the autophagic activity by altering the lysosomal protein; (2) up-regulation the genes in the pathway of neuroactive ligand-receptor interaction and promotion the function of neurons; (3) promotion the conversion of tyrosine into neurotransmitters; (4) regulation substrate feeding into the tricarboxylic acid (TCA) cycle and promotion free radical scavenging in neuronal cells; (5) promotion the fatty acid elongation and preservation neuronal cells avoid from oxidation.
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